	   SnapGene      0GACGGATCGGGAGATCTCCCGATCCCCTATGGTGCACTCTCAGTACAATCTGCTCTGATGCCGCATAGTTAAGCCAGTATCTGCTCCCTGCTTGTGTGTTGGAGGTCGCTGAGTAGTGCGCGAGCAAAATTTAAGCTACAACAAGGCAAGGCTTGACCGACAATTGCATGAAGAATCTGCTTAGGGTTAGGCGTTTTGCGCTGCTTCGCGATGTACGGGCCAGATATACGCGTTGACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGAGTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCTCTGGCTAACTAGAGAACCCACTGCTTACTGGCTTATCGAAATTAATACGACTCACTATAGGGAGACCCAAGCTGGCTAGCGTTTAAACTTAAGCTTGGTACCGAGCTCGGATCCGCCACCatggccttggctcctgagagggcagccccacgcgtgctgttcggagagtggctccttggagagatcagcagcggctgctatgaggggctgcagtggctggacgaggcccgcacctgtttccgcgtgccctggaagcacttcgcgcgcaaggacctgagcgaggccgacgcgcgcatcttcaaggcctgggctgtggcccgcggcaggtggccgcctagcagcaggggaggtggcccgccccccgaggctgagactgcggagcgcgccggctggaaaaccaacttccgctgcgcactgcgcagcacgcgtcgcttcgtgatgctgcgggataactcgggggacccggccgacccgcacaaggtgtacgcgctcagccgggagctgtgctggcgagaaggcccaggcacggaccagactgaggcagaggcccccgcagctgtcccaccaccacagggtgggcccccagggccattcctggcacacacacatgctggactccaagccccaggccccctccctgccccagctggtgacaagggggacctcctgctccaggcagtgcaacagagctgcctggcagaccatctgctgacagcgtcatggggggcagatccagtcccaaccaaggctcctggagagggacaagaagggcttcccctgactggggcctgtgctggaggcccagggctccctgctggggagctgtacgggtgggcagtagagacgacccccagccccgggccccagcccgcggcactaacgacaggcgaggccgcggccccagagtccccgcaccaggcagagccgtacctgtcaccctccccaagcgcctgcaccgcggtgcaagagcccagcccaggggcgctggacgtgaccatcatgtacaagggccgcacggtgctgcagaaggtggtgggacacccgagctgcacgttcctatacggccccccagacccagctgtccgggccacagacccccagcaggtagcattccccagccctgccgagctcccggaccagaagcagctgcgctacacggaggaactgctgcggcacgtggcccctgggttgcacctggagcttcgggggccacagctgtgggcccggcgcatgggcaagtgcaaggtgtactgggaggtgggcggacccccaggctccgccagcccctccaccccagcctgcctgctgcctcggaactgtgacacccccatcttcgacttcagagtcttcttccaagagctggtggaattccgggcacggcagcgccgtggctccccacgctataccatctacctgggcttcgggcaggacctgtcagctgggaggcccaaggagaagagcctggtcctggtgaagctggaaccctggctgtgccgagtgcacctagagggcacgcagcgtgagggtgtgtcttccctggatagcagcagcctcagcctctgcctgtccagcgccaacagcctctatgacgacatcgagtgcttccttatggagctggagcagcccgccCTCGAGGACTACAAAGACCATGACGGTGATTATAAAGATCATGACATCGACTACAAGGATGACGATGACAAGTAGTGAGGGCCCGTTTAAACCCGCTGATCAGCCTCGACTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGGCTTCTGAGGCGGAAAGAACCAGCTGGGGCTCTAGGGGGTATCCCCACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTAATTCTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTTTTTTGGAGGCCTAGGCTTTTGCAAAAAGCTCCCGGGAGCTTGTATATCCATTTTCGGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAGCGGGACTCTGGGGTTCGAAATGACCGACCAAGCGACGCCCAACCTGCCATCACGAGATTTCGATTCCACCGCCGCCTTCTATGAAAGGTTGGGCTTCGGAATCGTTTTCCGGGACGCCGGCTGGATGATCCTCCAGCGCGGGGATCTCATGCTGGAGTTCTTCGCCCACCCCAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTTTCACTGCATTCTAGTTGTGGTTTGTCCAAACTCATCAATGTATCTTATCATGTCTGTATACCGTCGACCTCTAGCTAGAGCTTGGCGTAATCATGGTCATAGCTGTTTCCTGTGTGAAATTGTTATCCGCTCACAATTCCACACAACATACGAGCCGGAAGCATAAAGTGTAAAGCCTGGGGTGCCTAATGAGTGAGCTAACTCACATTAATTGCGTTGCGCTCACTGCCCGCTTTCCAGTCGGGAAACCTGTCGTGCCAGCTGCATTAATGAATCGGCCAACGCGCGGGGAGAGGCGGTTTGCGTATTGGGCGCTCTTCCGCTTCCTCGCTCACTGACTCGCTGCGCTCGGTCGTTCGGCTGCGGCGAGCGGTATCAGCTCACTCAAAGGCGGTAATACGGTTATCCACAGAATCAGGGGATAACGCAGGAAAGAACATGTGAGCAAAAGGCCAGCAAAAGGCCAGGAACCGTAAAAAGGCCGCGTTGCTGGCGTTTTTCCATAGGCTCCGCCCCCCTGACGAGCATCACAAAAATCGACGCTCAAGTCAGAGGTGGCGAAACCCGACAGGACTATAAAGATACCAGGCGTTTCCCCCTGGAAGCTCCCTCGTGCGCTCTCCTGTTCCGACCCTGCCGCTTACCGGATACCTGTCCGCCTTTCTCCCTTCGGGAAGCGTGGCGCTTTCTCATAGCTCACGCTGTAGGTATCTCAGTTCGGTGTAGGTCGTTCGCTCCAAGCTGGGCTGTGTGCACGAACCCCCCGTTCAGCCCGACCGCTGCGCCTTATCCGGTAACTATCGTCTTGAGTCCAACCCGGTAAGACACGACTTATCGCCACTGGCAGCAGCCACTGGTAACAGGATTAGCAGAGCGAGGTATGTAGGCGGTGCTACAGAGTTCTTGAAGTGGTGGCCTAACTACGGCTACACTAGAAGAACAGTATTTGGTATCTGCGCTCTGCTGAAGCCAGTTACCTTCGGAAAAAGAGTTGGTAGCTCTTGATCCGGCAAACAAACCACCGCTGGTAGCGGTTTTTTTGTTTGCAAGCAGCAGATTACGCGCAGAAAAAAAGGATCTCAAGAAGATCCTTTGATCTTTTCTACGGGGTCTGACGCTCAGTGGAACGAAAACTCACGTTAAGGGATTTTGGTCATGAGATTATCAAAAAGGATCTTCACCTAGATCCTTTTAAATTAAAAATGAAGTTTTAAATCAATCTAAAGTATATATGAGTAAACTTGGTCTGACAGTTACCAATGCTTAATCAGTGAGGCACCTATCTCAGCGATCTGTCTATTTCGTTCATCCATAGTTGCCTGACTCCCCGTCGTGTAGATAACTACGATACGGGAGGGCTTACCATCTGGCCCCAGTGCTGCAATGATACCGCGAGACCCACGCTCACCGGCTCCAGATTTATCAGCAATAAACCAGCCAGCCGGAAGGGCCGAGCGCAGAAGTGGTCCTGCAACTTTATCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTCGCCAGTTAATAGTTTGCGCAACGTTGTTGCCATTGCTACAGGCATCGTGGTGTCACGCTCGTCGTTTGGTATGGCTTCATTCAGCTCCGGTTCCCAACGATCAAGGCGAGTTACATGATCCCCCATGTTGTGCAAAAAAGCGGTTAGCTCCTTCGGTCCTCCGATCGTTGTCAGAAGTAAGTTGGCCGCAGTGTTATCACTCATGGTTATGGCAGCACTGCATAATTCTCTTACTGTCATGCCATCCGTAAGATGCTTTTCTGTGACTGGTGAGTACTCAACCAAGTCATTCTGAGAATAGTGTATGCGGCGACCGAGTTGCTCTTGCCCGGCGTCAATACGGGATAATACCGCGCCACATAGCAGAACTTTAAAAGTGCTCATCATTGGAAAACGTTCTTCGGGGCGAAAACTCTCAAGGATCTTACCGCTGTTGAGATCCAGTTCGATGTAACCCACTCGTGCACCCAACTGATCTTCAGCATCTTTTACTTTCACCAGCGTTTCTGGGTGAGCAAAAACAGGAAGGCAAAATGCCGCAAAAAAGGGAATAAGGGCGACACGGAAATGTTGAATACTCATACTCTTCCTTTTTCAATATTATTGAAGCATTTATCAGGGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGGGTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTC  <a   (   %      -                *            %   %   6   .   %         #   !                                 #   (                                                #         -   6            B      %   1   %   !   "   <   "         0                           "   (   *      !               0                                                                                                                                  '   %      .            '                      &   (      
                                       	                                    '                *                                                $            #                  
                (   1   '         <   %                     #               7   !   %   2                        $      !      +            9   5             3            &   7       D        ( šÿ‹ûüÄÅCdèéŒ
�
Ž
“
˜
§
¨
É
÷
 ¥¦Ïw:wù„·ó%  š1›½ÙoŒñý$Æ*e>Ô©
²
Ê
vV[oÜúW…Ü¬K¶ƒ · °ppÕ'	�
”
+³åÒ}Íx¸]Ë- Ø �¿ gt}4DXtŽ#pê÷�»Â	ß	™
Ô
ø
§ø˜Ð,kx;ÈšL°ô‹ 	 d -´¾pÖå�­%%Of¹àÕß~	„	ª
!ïû�†­  .»¼Tˆú;
b
³
é
IÁØ W£ÏX7½:ÈLål„”  ‡œ ò6	É	­
Ë
w.@Fa}	"s¶èûêÃ—Ýø·Þ ›2ÚAþC˜Ç+?¡#Ç–	ž
7\+ÖâpÝŸ*  ±ey�q¼qó,8;…ñ(	©	?
|
�
•
Ñ
,1æÓ~ô ÎŽ4Ýó‘m^ E¿”ƒ.Øí'}—¼ÒFg¤ ¸ mŽÖ4´ì�mØyÄ2wŽõm¹î-Ì g ë`ÃÃ:¹ä@û% ½ ‚0©ý æ
(Ñ-l»^n†Ó
¸‹›ÑêP‘°ÚÉ•«õŒ% � Ï@sÊÀu>OˆÖë	uœÉÇ‘–Ã	š
Á
Éð8™y¯U_‰3p6 • U6Q}´Ýô
h¡·5Y,¡¼	Õ
Ý
ù
Œ¨ä,§ò†Ž©Âa­¾Ñ<WÉîÉäË^M±æ©. Ù L®Mbn|“8Uu~§ER�®´$ZqÎëøØà	ù‡ÄÊþu#ªÅy'2Dö½% e ½ýKŽŸMS(P¬ºÖ	%	”	Ý	`
«
ä
ÂÈíðüD„q‰ŒÐ]® 
 ?q»×æè¥3&|à:‚œ£‡êÖ† n .µ¿Ù»r	p	w
"a#ŒæA6TÌpœ# y -šG®&âgÍúG™á€å	…	šÚùý2k²ü'ÔoÌaÚ! © /515§âô<
´
ô<åQXo/¾î* 	Mm¾ Y¼c
ê
Ù!žì¤~ÀØY”;�Íæø•õ ¬ ‰´Âü—Ðt ˆ…�ê A½Uûî
JX J8YÉÎ 8 Ñ héÊ	Ð	Ì
éA^²±
#‰é\  Þ ˆó7	ø	©ëž§Î¸ß ð �¬	®
ºàmx�ŸTb·=¥«Ä„Þìí    ¡êµ	ò	/4G~Ì&tìüP˜£«ùnÃÕ:   r~§ú´þQ~g	¦	Í			)E,k^�Þl¾) ž ê ¿ú,¥@\j	»	Ó	Ÿ
·
<¢ã›œE� ÷ œ3“Ûd§I™?k—	Á	ü	8«”]¿qµ ¡Ži# š Yd)sBÿDÈï�cx¢$È 	5
CÑçëá×ƒ‡¦ÚkØ˜( ²…Ûlz‘rr9<Ìõ†Ÿ‘
–
Ò
/2ö5¿Ö¡ÃÑu�ñôÆ� fç½Zò#	ª	Û	Açë}öÖ%[gn_ ] _ Ü)	+	}
-ÆÈÔ»ÏÔ5Q™’�  s Í'+ÚJô-
		r	 
*
@
Àõ?r%cÉSÞÄâÙ.  p y„Ø	y
óè$7×Gh›Z FŽÀë»î=~˜½8VX# L �·ÞN÷? ¢/Œœ•Ù(Ó.¥�î * Ô Óì#Dw•lÒr a ç ×


HëÅr<JC‡Ewzßžš C ¹ ü 75�Î3Þö1×IeÎï!.Í À n�ÁÅ„å	
KÕxÍ�S³nvl W 0…ÒP…µí*?�n½Ù0Ocz
ÇÕ0>bè|]ºì h èßz£T`Ä;–� uªýT· ØÍ
–ÊXÆâK†Äˆå”
L~® ¦ t-	s
a˜‡ºAs:Ó b½lìQ	N
$Ý%eð|òŽü# kŒ	òs«ïš²ñÅ!Ï
ç
&)T¹¼Ôm÷‚ŒÏÖëÛkì“Ü w Ï I1ª²mêûo‡�Ò’ÎlÔÊ–ö� ¾ å 5ƒ‚Ë%Ýþ!	?.é¹:“HQ±	¬U- u Å 9;TV°²ÍÔæ	°þšV	$:T\ÊÒ7A_aEõ?€œ½Ër ŠpÁ6 ÐAt*Áïv…ˆÖP\×ì
vÊ2€ª—Ä	;	>	‡	›
¾
<íñ59¿jÌ9zB&°³É~�Œ4Ë { ‘ =Üö£
ûC\´?!,VzÉö#räcq{ > ¥ X‡ i
n
Â
 HLPÂô4cGE\jlqŠ<BÁ ! Ë2ÏÙ?‰�Ãì(LÈÚ’		ÊfšÿU`70B k Ç VzÏõi¢¸Êì(-_•¹ä÷Lai/Ii­ÂòL‚Ñ÷	�	ï	Æ
ô
œ¨ªÃ <b®¿ÊþåÊiºÌM!INo'dÆ † Z7~ø²«GÜ‡�eÒÛï®)àPƒ% Y – ² Ê JR�â6ã½t	Þ
k�ÂóyÈ�®'Ê)´L¦ãR²ª_1 3 R /œµÞÐZ›-¢Ö
ú
b›©å-­A“=X7åAž®¿ß_‚ÁÍB"JÁßç™´ó¡Û% ; N}´!¯Ï%–œ	‰~ÅËvµ¸t™G”«Æ4´áþEàHx! H Ú “ç¦rì2X	

B
P€ú$CS›ez(3©¶-a}QÃ"" Q¯c€çFèDHOçA	G	M	_	÷	
,
ˆÎÔÿŒLV·öt÷Š/< #M]kÇðöÄ)o”˜9Vv¡¨³S‰�–µü[‘Ïù6ÙŠ	Ž	á	
V^ jv‹• Ô÷Ùg’Hdg¾§" ¶ ™n¾þŠo|Ã)3W"	&	Þ	¦
ÿ
²äuÑÎZ9Û¯‚ŠÊ @»ÆÞ•	È	a
�
¬
î?E|!rá6žÂ–÷k f x�7‚>
{
lÃ�&9Ñ0 � ” ¼ La 7=NT¦ÕQ¨­È+×À
å
ãï+7ÃÉñý…V]Šé&1C]¯Ù^ˆÈo ,>¼ØçRá'{ºÌùqä	Á9ƒ1±ˆ‹Ëå@S « @404¦áí
ó;ä�ÿ
ŽŸ‡Íô  Ý é´	]¤¨ƒ¢ë�ì[ r¦Hî}bwŸ	¶
;¡æ›× r ÛÚÙ		q	Ú	¿õ4êq$ðÅZÃŒØ ) K o �¶Òë"Cvx×	x
ö>ç¡-7UYq B û /À	
Í�b	q/ç0BH†\dkÍ� S¥¼s#Ü$WdS‡�9}" ‹�ñH¯ýå™ 	U	#>S[ÉSéúèDlŒôÕÍjkëTÛ( z )î1„‡Ø†©:	=	†	¢
½
ìÿ4G[þ3>D[ +p²È}€õ6ÊAbÀ* … .y�›'^¸ãhHhš¬âñ�Ðö€	ó
š›Û;(­³É(hÞÀHIO³ò G "Ã  ˆ•Îû �5Cæ@	F	^	‰	ö	
Fýfµ, ! Ž “ ?`6Â2!6ÝÇ	=
¥
tY\Âè%0B•Úö
Çn�‰ ª /ø~ãž	³	µ
ì
8\å0§š?ž¡Z Úê¤Ö	Ûõ#=æp 
†ï,X|× F „ (xŽ0Ÿä¨õ"™þFRè‹=Z¿G+|Nê¿ �	²	ë
Úý"EŸÁ<Y	ç$/A¦>”õ+éÖ€0 } Ðã½[
×ØÙ“”•§¨¥#.?@xy<=�‘’“ÎóôæçèÕçùefs}~ö÷ ~ H	ÑäZ\
d
 –©¦8LUÙz!t–ø  Âˆ¾é	ÚDí€Z•X\Ï:ûèúgü I{­ 
~ºå]
—Ãý¶§€/‰ qOËu—ë x–_X
º
1˜9AVðøh‰±»†žµù  ¡ [Še
ªµ¹S¯MÚë6A@ . ­ Ò¡¥Ñ½u{"&V a�Ö¢¯}ÊtÀé ‹ íÞ[	KN¤Yžîõ¿Yhý >Ãð:‰Ãö¾VÛEp…K�,€ûÐ;¡%Ïû6 B¿üê	ï
U^–[9–Z’]üx%  +J®/c»¬|	Û
ê¥_«„ž¨0Á§+1 § |'ûe	¤	¹	Ë	Ñ	^
˜BR’þ$�¾ê!rè4ÌÓØ Ò ƒæÄŠ–ÕÝ!˜ì 9 åiê“Ä
Í
ùAJ½³²·eŠPO]‘v @  ‹—‰l8	»
2bæBÇ[„iŠÈ <y&ô°	Y
r-:W
¤Ÿù>¹‡ú ß ')ã$"™ù02¶ä á `V®Äžøñªì¨Ï²¼Ÿ¥à ¢ í ô o¤÷±ûf
p
¯
ŒUchû#Û7—B€â»Ð ñ ÿ P]\­	á¶T3nÅ…í>¸ € ¹ž»ny‚ «°¹(¦N^ßwîÃæð ‚‰‹‚
‡
eº™ßŠÝ¸>¬lì¯A�  WVm	¾	k
¢ýH¦ñö¼1ISÒv|„¦Œ 	×à{Óë¶	ó	5¨6›ÞžG†“¾Ûsð#WòÄ$ßÇ   / N ® HZóe³
[N0‰'uýŽ*™¬ú'fo¹;D  ¢z$¤ÍíáùyQ¤€+5ÖT  Ö C½˜×An*X·”§	Ç
*l,óÿµª´’ bb£h	Î	
'-_¡úmWŸË¥Á s¨ûLµNÊƒñ(FÕuê* ã ‘îÿÉRÛ©	8°hÆ�ß~€ˆ¿Ý— mßû¦uï\	 
=ŸýœÀãF†i , ‰ Œ Ÿ îÀ-›	Å	Ô	¸
L¥Zœ£6JSéóvZþ ë :Õ%Fy‘k	¼	ïäö1ÑÆâ ‹��Aµ]JOñ�� ÄGN¨ÑJ@¿Wý	K
F•N^LÀgE²¬¹9Ij7 ø ;iŸ˜	Â	¬¡�ü¢ ?�Ë4<ÜŠ4ÄlLÜ¢<&ÐK # ”ñe÷šE
9Qq†r‚-¶ãÑ�'tü*¡§ › Z—îDèØ„± :çlaÑã™¾ `Í©ÀEgjðÅ£°¡	ð
_âåˆ¬^L¶ýyQ *CÉÔÜ·É	ÌìŽÕ—§\î4 b% e¬Ct 	‚ÛCdy%>_ýë	6
ÒV€ò—Û[“Ù&2' ¯0€¼fsBcs=ÔöºÓ‹
’
—
±
È
Ó
ö
÷|—jÌvÇ™K\% c ,³†ä-º—$Nß‡ù5	}	ƒ	:
 6HÀ×ú`€*¢µç¼Çä  d“»:„êð03ëŽŸ1vÂ»òEfã?�£,l. KTÎ?rÉ5P|­³Ü	m{’/‡êÍ 	Ü
¦"`¬…©ÄÒí	·O�®õ¼¨2 m ºüF¤L$	Ü	_
v
ã
™ÙìCj"&‹é5\o…`Ù ¨ óWûÿP ¿×sí)œ½é 7 ï gè«	ñ	ßèlž3S±°Ë%÷ëOªÍÔ9' ™ é ö Xq}¦ù(’³ý¾ù>[f	¥	º	Ì	Ò	4
Bª“~]‚´k½D�h( \ ^ k*IôY	*	ÿ	.¾ÅÇÕ>ÂÐtÓÕPRáf Ó Ýk‹›#6ÖšŽ"í ` „ÄÑ

>¼NÌÝÔuDyÞ ™ ça×kÌ,	P	M
r
~
ÉÅáïJ…{—_ñr“	KÒ t Ä jªÌÓî¯ÄÎ
6¸9>’¼ÎÊqo’    ¤ Ûëõ1KÙ	h
úBK¾
e³Á%ikTyÈ"ã;/& 2 Q X ± É IÎ÷ë,”ÏöK`.ÁKs	Å
Á¬@d’�ý&Lß&QÅ‚^ Ú( : Ææ(²æ•GN	L	


+
A
O}Ó´ös˜Øf‹“šGdß‰G|P¦w! µ » ˜mz
þ
±ãÐ\%8Ø
 =z	°	œRó A q ú ŒRÙ	
¾ô3Vcép…[j‹ !Š€ƒ…9	¼
ZçC¬ÈÇHiñÉ E ƒ '�!s™ÙWYˆ GÁ‡±	è	Z
Ÿ’;X>¥ *9úÔû z^W
0¸®.¼5pïU@g?º =�ì9õ¡£®o„+¾ú $  *‚ä.úd	{	Ú
ø@¼‘½Ô3¦0� à (#%kU­¯	q!,�£/1 ó þ ¸öO\†
d‹¸Þ‰gú"–v=·ºÏåï M ßUZ#£šÝ0R�’àÚø)xƒ¥ïeñ*#Æ Õ â B¼íKamW7ÆÅ+ùòþ‡´¤©³ + ˆ 9Ô$Ex´š	Ä	I5â…Ðá	 ;MHsJ ¦ _–Ó¯¶ÔñŸ«æí3KµÐ½
 b Í>qÈ’²ã°
�ù{iìÆ¶˜Dâ>¢ã ˜ è î õ p¥ø‘²ü3
ÞkVÊ\ŠìN©›¼è [ Ã
q
=àIszÜtCxàÑ £ Ã Ø

g
N|	d5‘’ü$8=‘KxˆFn{�Ÿ¥ ´ º Q°½âÏUÆðò D ‚  ]†Œç	 /‘¢­·4oØT?fÓ ò ý -Tz	®	p·»!+‘ß÷w¤îð)"/¹ÅÎ 8A»^L6Å*4å2†J´ÏG	I Â p�ÂÇ
j{øßy�sw¨ám¡ç � º…æ	¯¼Ä.o¡¬¶º) ï(ÒHÄñ' Á oÆiz±²³´µõö÷)ÝÞ°Ö_`abTUopqr¯à
aG¯°  Ÿ6ŸÞ R
gmz�ƒ*L±§×OcÎNV´ù°xbMX± Ì Ó6äåh¿h†„+M‹ÞP?{¨ÌÈ1?7y^øð ]nî¹Pdcý}¬°>N² w
ƒ
ˆ
à
n{+f»šoÚ²OYdgºmúé±Bc‚íY* 1† 7 ß!Æƒ8QŠzŒ0	v	/
S
qv�¶@‘à¾1­ØÏíO¢¨WµÖ»«	  žÔùæRÀ£^…,Œ§ÍUwÁÉ28ùñ Win	’	¿	ú	ÏCß÷½|©¤} éåIx{#ÓaÅÇ<…z—�›ž, i Í X7àl
i~‡þNò?Q2@JT¬¸@§_ ' ìoÎD	ô	ËYÇ7ßÜ‰-dÃXió±?È 
v«Øþ|¸Ô^—S	Q6ãZ‡å„œñ$Åv¯ ³ áU·	€
‡©œº „”¿Åæ~­)MÝà£Ö –gÙïLŒŸHQeç/C°átÓþá•%O 0 5 O ¯ 3ôJ	
t
‰
á
¼Ñ×1¯(³ht™$»BpºdƒZ I!´b	
„
Ø
ü
�bP»nª²(t; [fux.	o,;gÛZe� ˆêL­ûE£î<E©  % §1|\O›p€ŠvþÏû+šgCþÔ 2ó£"Çm/�·sˆ’�r¿ñÎÙâ£a×ózR,¶ U
 ¡¡©àœÒ;{R	%Aá%¥®ñ}îPúXD��é26 8·à„Ì!Ríw	0
O
T
dw2‚• îÐ©Ñ¥õý¬$   T ‡c®9\¾ß‹�¤1	$
G
r‘«Þç&/f{9¶Ãý‘¼×  × ™Øðú#o�oç�Á*¤+UvnÛøVƒ¶«Jµò  ŸÕ¬B ¸"Sè
-ÕÎ é9“Ý D¾l�
ò+¨	Ð
_×ó-�Â×ìô3|–Ül¸íú# ót¶›³YÆü•'‹È†t�¨Áº½ÐmÈãxÊÐ Tå”ª x ™Jž2«F˜
	o	“	Ç›øüp`¢ûÓnÏÕË› X³në.I–}“XÌ÷  Ð Ï	¹€ˆ<ªm¦Â � ccP€¤ji	À	û	Ðê(Dàj¾†Ž¥~šÙj —Ž „Ì&æËž"	)
@º|õ$bÈ
˜Ž-
 €MêÑòº”<W ¿ æ 6ƒOä	„)J/GêÔ;ÆIR²=aÖ{vŸëV t©ü¶Þÿ•y¢†•œ­+  v Î ä 4Ê$8:SUr±Ü™±ð%9±@`ö�G‰ÓÀ =  <WÎÕ[Â'ÿ	m
;OiË8óbAF‹qz( j Æ YÉá
±ªî	FajxÇ­Ú6ä@K�¥­¹¾�Ìâ An!cÀÞ˜1 P\j’ïõ¥—³ç1›W	�	U]ˆÿiu~Š”ŸÍ·ÓBR3KU‘³¶àcõs¨.`Â' w‰<n{¥ÔV§Ç�*Åœ
¿
âî*6>kÍ{ 8Uà5�®Á‡jÉ Ü +GQàí¹Ë3av¥pò:Àã þŠÊä‚�ëÌ ( J Ñ!BuØwÌŽR./ÄGYÂ8c< Âíð†×]·ü‡”ÿg~�«ð4B˜Ï	<	?	E	T	]	ˆ	õ	
¡
ò
=Rë3ZÈ:ECó'gÝ*e±´jôÿ²5@S% � ’ Ùé£w>_žÁ.ã÷ }ÜÆ	Õ	¤
˜7[äç[Š…F{�Æ{ | Ïâœ	ÖC¦üD7KTæ"-wW[òådr|ÿ -   w•¬ÿ}¹¹

R¤µ@ˆê%NÊ÷°´ Š `ÕïÂÝZ	MT]�•ô|‘$s¿w5 ¦ &b«’£	¸	Ã
I¤Qªdƒ�•ÀçÒÜ*N× ? &;ŠâaÃåÆ÷Zƒ=Ç¤ã ì n£°ú�ˆo
�
˜'Ü]k2m®€áÂ# ÖGVzYòd²l	½	
j
üMyˆ§ðõ5»H�F…r¸4‹SÞC —ÈÚ@)M¶É“¨	‚ð'g&  VžÔÜ–‘ lŠŒŽ�tî›IRèðò0üuÅ " ÊFh3ÐžD
J
KPMfD±â«¸&8) «Ì¨fiÄÚíBÛ=^	Ë�ä°`ÿaâ1P$7 S4O{,e¹.†–ºé#AMbÓÞƒ¹Òéïø 	4	‚	9
Š
Ç
õ
G–!_)´ËuæN»J­ºe»k[! 6 l W¹E£Kø=ð	u
â
�©Ø2°úO¯ÿi³j(¼CŒ„8'% Ðj)`HjÖjóXË	O	þ	
L
½Äî=„MÁšËÒ^Óð"OqJe2 1 P W ° È ÅHö©ËÒí±Îåõ_ê0J”®ÀÃFJMK	Ò«c—À×Ž™hFj»ÍÞp%Ç!: ù <‚	
ý
Yo«¯Q 8 V
žnƒ­*ù)  õ["jùT¬c	…
Ù
YcŠÝˆ�fÜQ¼¢‚du< ‡ :J³µ™	Ã	HÓðáý³£„ Z — Ì=‘±â2
MÝ<z4�É‘[µ‰ëM=¤ ³ @,KS‹y	*�äv£S³F ž5ÝQ
flyŽ®Ã-hôÜ¯(Mø§®	'`ÑFL®$ Ë 5\Åã7ägmvy¾/	u	
.
ß
puNXf¹Ë¡§ 6ü«÷ï=ª �øQ‘	ù	}Î]B>w"«·ÀÆš+! & •fC	RP‹Yÿƒæ.B¯àäƒ,›ÂÒhà(uÜÿ¢Õ $ 4 02I	a	×
û
œ:ÐÖ®s©ŸÎ#KD¢ý¨+  S ¨ò¶ß›­ËÑ [Þœ.:£#
F
cªæ.r‡œq�”8ð`ÂÐüC�è1µôÿ µ"ŒnŸûŽŠsÀÖòuÏÇÚâÿè{•SäI·©  œ ˜O�E—		¸ÆéšHi•;…™Øiš Ð�ã	(
”¹ô¡;`”V9 < 3O[iô¤#q~–ÈZ˜°Áï&0Œ	í	E`ht}‰“žÌw¶¬Ù5²µãJ¤FbáômbÒ-¿y5 I Û  ;Fz¤¶ÓU†“¸Æþ`f}Ž¤)3AS	
ñ
Qáê)2�âDTòQf)4­.Áêþ7bR _”è¢ÎávÁ�â|ÛY	ÕBæ‰”~{E�M¾z4  %m‰¢a¯ùª€ñc±¢	
û`x—£&¦Û‚œY‚·3~ÛR ÉªE§Çehksí<§C
I

�&%ã¯ïûª·_ÛÄ(#3 V R¸+Dd…°Íè"<G^Õé“ÒÝEI¸Êèîÿ3	N	8
íùƒ–Ö�˜Ñ]iºÒÝï‹ &  ô9�S	U
XX‰m‚ øc +°áx	
-
1
P
etx­3�MW¸÷ÿu5¦ö­ ¬ÊÝ9PB	H	`	�	ø	"
ÍÏÕ!ï¨ªÅ0þ&  Nhp•œ
Y‹—ÀDžú‹	â	ì	'
_“g|Õhó:I˜_h”“¨7 $^lˆÈ®øDuÆ:dy£µT…’·áý_e|�(@b¦ÚH
	Ôàé(1wŒ–¢Ú�“ÀÚ½Ú'3   U ó*«¯;]Ü’Ñ8þ2	�	!
7
Ws¬Wl�øh¹ÄÜþD  fgñò­÷Å™š›WXwx¢©ª´:ƒ„Š‘¶¿à\]^z{ŒÐ&'7?`a¥ 
%
&
’Óßè'0k€¡efg·¸\]^’¿ØÙ  3à  ›GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                        $                                                                                                                                                                                                                                                                                                                           :                                                                                                                                                    	                                                                                                                                                                                                                                                                                                                                                                                            
                                                                                                      	                           
                                                                            	      +                            *                                   
                                                	         3   	                                        
                                                                          	                                       
   
               '   )                                 B   /         	   
            
   
               
                        	                           	                            9   #      *   -         .         «   S          †   |         %      d                     $                                                                                                                                                   :                                                                    	                                                                                                                                                                                
                                    	           
                          	  +          *             
                  	   3 	              
                          	               
 
     ' )           B /   	 
    
 
     
        	         	          9 #  * -   .   « S    † |   %  d   u…  w  w1  w�  |³  ‚…  ‹“ckçk|lð$Dp
:FhkÍ":;<]…†“Àýþzû|èWž¹Ùò{);áøŽíîì(ñ9!J ðÅE	´úE/1´æè—&kMÇ¦v%è×ÈkAA!†ïûCDÍ¾ MÇçkƒ¦ï)2¢«ÍÔÙ(VvèiÚã-¢>	M	V	�	Æ
Þ
Gb€ûCvÅËÃ.¹¿4X‡’?“ÖH�ªí±}¤/~ŽØš%	w
~œŽ~Ž~š~/bŒíî£	ƒ‹*‘¤)á)4ÎÉ"u§a�	èëµ	z¤è8Rë µ	4& L„å	�	+vB	DB	² žâ/Õ@ýTt¦ÆCŽÆÞ	"ÝÑ/ž,1 ý ù:yTÑ!�Ù!@ýÆÆ®	p+aýr§ú´)œ£,ÌÓ6oÕ—v€j”qÊ”?
ÉÑô_`5ãkÔÚté{t"©Äx~ ‰©LOÿ(m=Ér§ú´Ñ�Þ«)‹ã ÉÛø  -š™	?ýSÙ. 	S.œø‹D‘	tŽ÷ß	ÿ \¶3n> {~ 'tŽ÷�		ß	˜õS." -štXŽú™÷ß	Ô
ø
§; "Æ+Ñ´
t-	Â
 Hc¢êÞñ	3@•ŒÀj0"á„å™U	S[Ñ› Ê” ´ÝýþUtu§ÆCYŽ�´ZÎé÷#,¡úß	?
¨´Éä,Ñô7^_½æ¹#MÞ‚	Ñðè‰Yƒ›ÿqÖKÉð aª°á*dq�:yz£TUŠ‹˜°ïÑÒ(P�¸Ûþ2	7
à(£Æ u‚¹‡jñçz¶´É´‰Æáã9T°_ítµ…’ýe|�@é1aÑÆ
ô
ÊIêJtÂj—|aª£‚Í  -š'tXŽú™÷�		ß	Ô
ø
§˜õ?ý;SÙ.·	æÖœaª°áÛ£ ‚Ÿ¨â/w Mz~Ë%9To”°´Ù-–è2DH	Š	
¾
Â
P^‰í 5Hf û=C_cŒ�ÆáHeÒ(Mzaªq˜°ï�£‚‘Â
 HcK  .}sû—¢øÏ ‡Vj
üðH×FÐfD8‹q˜°ï…æ	Ò æ
Óó£/sˆ’�aó,-š™#MÞ‚	Oÿ(Ñmðè=í†~<	ë3±õ_ÀJ%‰Jâç £,Ž ç À	í$5œ£S8.,  Í ·å·	á6ã({³ß9ZŸUˆêLœæéÖ  ã ÉÛ×�a ´‰ÆáðqÈ]·˜°ïg«ðÏí	ò
:gmb–	Å™‘	0]·g«ðÏò
:g£,‹‘	Yƒ‰›ÿµš çƒ˜^Áù) Ò"Cvq5|3Ží1,  .F}sû—ø ¡ÌìP£Õ]Vì›²¼èR+d;z…¤Óè"UÆÒÝ)¸èîÿ3	8
á)T4­º¦Óq¥|:ÖÇ Áïõ…ÊÖì\ä/Iª­ÂL—	;	>	‡	<ª9b¿B&iºÌo°³~Æ4Ø LAn“þ8CUtu}§ÇDRŽ�´#Zp¡Îê÷ø#ÇØëß	à	øÖ½û"‹ŒnžúŽÕuÚ”I¨e¼ñ©	æm^ÙŒþáOAþCÇ¡#ÇëÖûÁï…Ö\ª—	;	>	‡	<9B&°³~4Ç õÊìä/I­ÂLªb¿iºÌoÆñc±
ûx¦œ·3Ré^
X”ÇlÿÏœ¸; Ç Áïòõs}…ÊÖì\äš¯²%/Iª­ÂÅL—	;	>	‡	&<Ëvªµ9b¹¼¿«4B´&iºÌÏEào°³~Æ4“xR+d";z…¤Óè"U‹ŒÆnžÒÝú)Ž¸èîÿ3	8
á)ÕuTÚ4­º”I¨Xi
Lô4GEqŠB8 héÌ
²±‰\N@F•^¬¹jI  �Q.Á7bÉ	Ë
@	"èy4S& C	,Âhl ¹E£Ku
â
Øi„š1ÙýÆ*>[oÜb½Ý%eC½*W§	ý	K

LÀ, ó:FI& l ³ ¹E£KC	u
â
Øi,Âh„ØÄYÂFÐJ
MfD±«¸8þQ�Þ¾Ýþ¬Nhp•
Y—À‹	ì	_g|Iï)Tm÷‚¬ “d™»r"8·=q«Ðtµ ŒŽPÞ(DjyŽ¢~•œ+.5 žÔRot
á
·×,1¯hÍ‰×8»B ƒ
-
tMW¸ÿ5ö515<
X	mR µÞÐZ›-¢b©å-“7_ÁBç´ó­%fà~	„	7Žë½ØZ5AK}3~½1I”Îg#© â´
/ [fux.	oe ûî<©»r"Œ“d™8qµŽ½ØZAK}”g¬ ·=«Ðt ; òs}š¯²%Å&Ëvµ¹¼«4´ÏEà“xFÐØJ
MfD±«¸ÄYÂ8§ þ¾r¹}¬ A·’Uv Î ä 4Ê$8:SUr±Ü™±ð%9±@`ö�G‰ÓÀ< 3O[iô¤#q~–ÈZ˜°Áï&0Œ	í	E`ht}‰“žÌw¶¬Ù5²µãJ¤FbáômbÒ-¿ykŒ	òs«ïš²ñÅ!Ï
ç
&)T¹¼Ôm÷‚ŒÏÖëÛkì“Ü* Ô Óì#Dw•lÒr… .y�›'^¸ãhHhš¬âñ�Ðö€	ó
š›Û;(­³É(hÞÀHIO³òu Å 9;TV°²ÍÔæ	°þšV	$:T\ÊÒ7A_aEõ?€œ½Ër ŠpÁù <‚	
ý
Yo«¯Q   ¡êµ	ò	/4G~Ì&tìüP˜£«ùnÃÕ:Ù L®Mbn|“8Uu~§ER�®´$ZqÎëøØà	ù‡ÄÊþu#ªÅy'2Dö½µ"ŒnŸûŽŠsÀÖòuÏÇÚâÿè{•SäI·©¦ &b«’£	¸	Ã
I¤Qªdƒ�•ÀçÒÜ*N×G … • Ù "LU.6Qy}�›®´ÃÝô
Mbhn|“¡· '8U^u~ §¸ã5ERYhˆ�•®´Îû $HZhq�š¬Îâëñø,5C�¡¼ÐØæö	@	F	^	€	‰	à	ö	
Õ
Ý
ó
ù
Œš¨ù‡›ä,§ÄÊÛòþu†Ž©Â#;a­¾Ñ<FWªÅÉîÉä(­³Éý(hyËÞ'2D^ÀHMfµö,I±½æ O©³ò ? ¦ à &(*;‚Šâä&.b#%k«ú’U­d	{	£	¯	¸	Ã
Ú
qø@Ia¤¼!Q‘ªÃdƒ�å½,•�ÀÆ÷Z£Ôç/13ƒ¦ÒÜ*0=N�Ç¤×ãs¨ûLµNÊƒñ(FÕuê*  ¤ Ûëõ1KÙ	h
úBK¾
e³Á%ikTyÈ"ã;/I V Û R ¸+Ddµ";Fz…¤°¶ÍÓè"<GU^†Œ“¸ÆÕéþ`fn}Ž“Ÿ¤ÒÝû)3AEIŽ¸Êèîÿ3	N	S	
8
ñ
QŠáê)2�âsÀÖíòùuƒ–ÏÖDT�Ç˜ÚâòÑÿQ]f)4i­ºÒÝèï{•.S‹Áäêþ 7Ib·©R&S4O{,ew‰µ¹".<n{†–¥ºÔé#AMVbŒ§Çn�ŸÓÞû*ƒŽ¹ÅÒéïø 	4	‚	9
Š
œ
¿
Ç
õ
ŠGâî*6>ks–ÀÍÖò!_u{Ï )8U´ÇËàuÚâæÿ5N�®»èJ{•­ºÁSe»äI‡·k©[jÉ ¡ [Še
ªµ¹S¯MÚë6A@¬ÊÝ9PB	H	`	�	ø	"
ÍÏÕ!ï¨ªÅ0þe ½ýKŽŸMS(P¬ºÖ	%	”	Ý	`
«
ä
ÂÈíðüD„q‰ŒÐ]®D ‚  ]†Œç	 /‘¢­·4oØT?fÓf … s¨û*Lµ.y�›Cx�'^¸ÉãhÔ7HNh‚š¬ÊÜâñ�·ÉÐö	€	>
{
ó
ƒšÌñ(›Ûlì;FŽÕ—Ã(§­³É(\hÞ�Àî&9HÑ4IÕ bOu³òê%*Win	’	¿	ú	ÏCß÷½|©¤}-  )+     ç  2        2¿þ`�îÒu¸îód¥=&ã¿ŽˆŠ,á$ŒÄÕjS�uC¡ñÖ�…ã¤
C*Œã¬Æ'Ñ™Ì¡Úhy)¥’[Zpi+dý™�+Ì§eéÂVÏƒ‡Ï×üý¡ËYaÉŠý’\õ�¯hrÖšœ%xýè4�µ"m˜õ'?ù¡ËXp•ƒóàÚcô�‡é€z¾z'nri-³š}‚Â¨4�µ€È0¨{p¯RïÚµ€JŠ ßUµÀz½V,—^óý¡ÍX$2'.fSDîàåäEýã§ƒ¦ÅigÞdSƒ“*W¥:	ñ;óß`úøD?ÜÈâ°Dƒ¾�´Ó9„� àqÍø«Ì�àSzÌÍAøLCÇ36ëPø üÃB>M4??üC„xÁ3<8}zóŒÞ3MÌ³¬†8Àeì ÍÔ"s;O8ŽEÐÜG„4Cÿ4âý÷Ýÿ@ö¾ÝÝŽ½Oô?ûøÿNu ¾;ÐÒ5Ñ8ø4}¸áÓ-€Fô¿_Pï�E¯øxâ8ý¿Ž N'IÇÿOð?ÓóåÇCóôKüßCDò~-þÿS>7Ì#Q?OÐàrm‰Ô7ãE üN7+æpÿôN/Ýý4$ÚÿOãÄïÁôSN3�ÁãŒ7 ÿ€£~ë =ÓˆÃ6R5žýóÓþ÷ý>>8ûâÿÛ¬zÑ.ºõ‚¯pÓ<ƒ¿Üæ}¹ø°4‹NÔDø/‚à_àâ<Hß‘sL„‹¯àdÓO´û�‹Ó=tû˜qÛ:ú`NÓóäìAçT}‡i•aÛuaÔ’?*Wó†Óìx�®J==¸¢£þÿÈúúàP�{÷‹êùeXAZ6Í¢á$"Úæ¸  AÓP€QÖMŽ cæ¸CEôàä ›ýÌþ$Ã6”Ì0")ÌÓ!ó�èôÏäÏÎú³«¯ëêì÷+<:¿ËS¹[ ;ú�+Æ¤Ž§ûõUèi+rHlþ*¬þ¡Ê½ÊêY {¢½^×{_æì.kª™@j�°ù ¥U„á©uV¥ÌZZâˆ‹J@ˆ/ô;ý44RcTØÛµ¤´×Ðˆ/ô;ý44RcTØÛµ¤´×Ù/Ïù{ÏÛóþ^óô¯?k¼ÿ`áåªªjcD<CÏ¸û„æ½’Cª Cä:£UNüè™½ªÁ¸mDtA²¬Øh]F�Í
ï:CšÃ˜x×G[;†<ò+Ã‹LÐü+ª‹Qñø¼#øX^4CLÃ› à÷¯£4â;¢ÞÀP9 1Hðï‹nß£¾<EI½¶Ö0Ã�œèo~?k}LWlÛr{<.‹A†àqDÛ@;3ÓÅâ³ÐíC3cñÃnË!ýƒøèñfØ UƒÎ«�­±â Áó›AÙ2ƒŸÈfŽNƒX:û#©Âló¿Í3lúæÏ®‡ºá0à
Åañõ1Ï×ãÛq±¯|óë˜T(°[*¼<8o½3 nØàJë?×¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸™òÇîNDè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘EÒj	¸Îã…=)úÁU‚Nèo]_|à“
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{gºú«Y¦…6¦Ð)¢í„Á�š…‰©Y]Y ¯3v¯ñT�~  ¬  	“  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  <?xml version="1.0"?><Features nextValidID="17"><Feature recentID="0" name="IRF7" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="941-2452" color="#ffcc00" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="16" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4832-4848" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="863-881" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4901-4922" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment name="-10" range="4856-4862" color="#ffffff" type="standard"/><Segment range="4863-4880" color="#ffffff" type="standard"/><Segment name="-35" range="4881-4886" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="3xFLAG" directionality="1" translationMW="2730.72" type="CDS" allowSegmentOverlaps="0" cleavageArrows="2524" consecutiveTranslationNumbering="1" maxRunOn="2527" maxFusedRunOn="2527" detectionMode="exactProteinMatch"><Segment range="2459-2524" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;three tandem FLAGÂ® epitope tags, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6827-6931" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4638-4759" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3454-3589" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="615-818" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2562-2786" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3274-3603" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="235-614" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2832-3260" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5210-5795" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="Neo" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3670-4464" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6757" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5966-6757" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6758-6826" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  @<Notes>
<UUID>a6e8cbc2-d930-4a6a-be3f-02fc170a5604</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.6.5</Created>
<LastModified UTC="5:26:10">2023.12.12</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  <StrandColors><TopStrand><ColorRange range="916..933" colors="black"/><ColorRange range="934..939" colors="light blue"/></TopStrand><BottomStrand><ColorRange range="916..933" colors="black"/><ColorRange range="934..939" colors="light blue"/></BottomStrand></StrandColors>  )z     É  º        º¿þ`�îÒu¸îód¥=&ã¿ŽˆŠ,á$ŒÄÕjS�uC¡ñÖ�…ã¤
C*Œã¬Æ'Ñ™Ì¡Úhy)¥’[Zpi+dý™�+Ì§eéÂVÏƒ‡Ï×üý¡ËYaÉŠý’\õ�¯hrÖšœ%xýè4�µ"m˜õ'?ù¡ËXp•ƒóàÚcô�‡é€z¾z'nri-³š}‚Â¨4�µ€È0¨{p¯RïÚµ€JŠ ßUµÀz½V,—^óý¡ÍX$2'.fSDîàåäEýã§ƒ¦ÅigÞdSƒ“*W¥:	ñ;óß`W¨º¿Ñ™bN¢Ù¬ÖQº]á‚U~ßQaº›Õ½SáK£v�„O§VI˜±à]ª�†=!{…-‰½Ú–º}­5©)í¸Ú¨ù¸ð!aêã†k˜½TÕpu,*¥ ÚmzšÞ´E6<…rf«¨5™[î0uX×JW<ÝôÚXW�èkŠô¡DÔQ+SN­´�u·•´­µUÆHãQZýTRÕš´E_S*•h°‚®¡QYÔD¨UÕZ k½JTZeEU…Fp½éU�GQH Ef¹�Ò‡��­‹J#JfÕEYÐ~­dV“Òn•O…è}Z!„´^V…U¬­Txa¢PUz=Óu­VVQM…¥5�\Ý%¡�eJÔUQâæ_u¥µ½Ui^�k]yTcŽáKôš¹^…^:�ŸqÂhU4Aï˜åE8^AF+U‹_ìAçT}‡i•aÛuaÔ’?*Wó†Óìx�®J==¸¢£þÿÈúúàP�{÷‹êùeXAZ6Í¢á$"Úæ¸  AÓP€QÖMŽ cæ¸CEôàä ›ýÌþ$Ã6”Ì0")ÌÓ!ó�èôÏäÏÎú³«¯ëêì÷+<:¿ËS¹[ ;ú�+Æ¤Ž§ûõUèi+rHlþ*¬þ¡Ê½ÊêY {¢½^×{_æì.kª™@j�°ù ¥U„á©uV¥ÌZZâˆ‹J@ˆ/ô;ý44RcTØÛµ¤´×Ðˆ/ô;ý44RcTØÛµ¤´×Ù/Ïù{ÏÛóþ^óô¯?k¼ÿ`áåªªjcD<CÏ¸û„æ½’Cª Cä:£UNüè™½ªÁ¸mDtA²¬Øh]F�Í
ï:CšÃ˜x×G[;†<ò+Ã‹LÐü+ª‹Qñø¼#øX^4CLÃ› à÷¯£4â;¢ÞÀP9 1Hðï‹nß£¾<EI½¶Ö0Ã�œèo~?k}LWlÛr{<.‹A†àqDÛ@;3ÓÅâ³ÐíC3cñÃnË!ýƒøèñfØ UƒÎ«�­±â Áó›AÙ2ƒŸÈfŽNƒX:û#©Âló¿Í3lúæÏ®‡ºá0à
Åañõ1Ï×ãÛq±¯|óë˜T(°[*¼<8o½3 nØàJë?×¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸™òÇîNDè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘EÒj	¸Îã…=)úÁU‚Nèo]_|à“
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{gºú«Y¦…6¦Ð)¢í„Á�š…‰©Y]Y ¯3v¯ñT�~  ¬  	  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  .<?xml version="1.0"?><Features nextValidID="17"><Feature recentID="16" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4712-4728" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="863-881" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4781-4802" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment name="-10" range="4736-4742" color="#ffffff" type="standard"/><Segment range="4743-4760" color="#ffffff" type="standard"/><Segment name="-35" range="4761-4766" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="3xFLAG" directionality="1" translationMW="2730.72" type="CDS" allowSegmentOverlaps="0" cleavageArrows="2404" consecutiveTranslationNumbering="1" maxRunOn="2407" maxFusedRunOn="2407" detectionMode="exactProteinMatch"><Segment range="2339-2404" color="#cc99b2" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;three tandem FLAGÂ® epitope tags, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="11" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6707-6811" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4518-4639" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3334-3469" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="615-818" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2442-2666" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3154-3483" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="235-614" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2712-3140" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5090-5675" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="Neo" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3550-4344" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="1" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6637" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5846-6637" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6638-6706" color="#ccffcc" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q></Feature><Feature recentID="0" name="NUF2" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2407" maxFusedRunOn="2407"><Segment range="941-2332" color="#ffcc00" type="standard" translated="1"/><Q name="transl_table"><V int="1"/></Q><Q name="codon_start"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  @<Notes>
<UUID>a6e8cbc2-d930-4a6a-be3f-02fc170a5604</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.6.5</Created>
<LastModified UTC="5:25:49">2023.12.12</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  i<StrandColors><TopStrand><ColorRange range="916..933" colors="black"/><ColorRange range="934..939" colors="light blue"/><ColorRange range="940..942" colors="black"/></TopStrand><BottomStrand><ColorRange range="916..933" colors="black"/><ColorRange range="934..939" colors="light blue"/><ColorRange range="940..942" colors="black"/></BottomStrand></StrandColors>  )P     é  ;        ;¿þ`�îÒu¸îód¥=&ã¿ŽˆŠ,á$ŒÄÕjS�uC¡ñÖ�…ã¤
C*Œã¬Æ'Ñ™Ì¡Úhy)¥’[Zpi+dý™�+Ì§eéÂVÏƒ‡Ï×üý¡ËYaÉŠý’\õ�¯hrÖšœ%xýè4�µ"m˜õ'?ù¡ËXp•ƒóàÚcô�‡é€z¾z'nri-³š}‚Â¨4�µ€È0¨{p¯RïÚµ€JŠ ßUµÀz½V,—^óý¡ÍX$2'.fSDîàåäEýã§ƒ¦ÅigÞdSƒ“*W¥:	ñ;óß`Q¼ÉpÏs9E<¼Å3?Ç4ý4@ýÌ8ÃóT ;0ÀÁ2ßpE=Èÿ@ÿ6ôÔxNßTctþÃØÃA=ó0øòð#ý4ÐöøÁ8üòpý<üÁ¿Ì,”ù=Ñ@33½ð4ý<ÄÐP_S•#�Õ¡ÍQ›°CÂT¢ÏŽ3E�ÀK­PˆNx4ç˜]ˆQ«Žùaj¦*W�Ký„çQf˜WB:¹º!ZÕE¯qo8KjéI¦ÓŸQŽ-R¤m¥E¥EmQ™´ˆAUôþ8—zé´øQ[¸�™kƒ¨ÕCNšIõEŸšÔ88ÕHAõ¹G<ªnÉýE�P`VJ­Aø3îêîÝáT�ª[pWcˆ[¯Õc�EŠ•iaYú ](UQ*ÔN6¸¯ŸbxöêÝˆÐ4- ôí;ã¬e(¤ÚêèHûAÑàm¦©¯T­nk»Qyh|UIQjæÅhY…Ee€«ÖªN»yÕaÂeFØvÇ�A‡u$„Ê•üá´û#ë’�On(¨ÿ¿ò>¾‡ø#Þýâú¾YVV�³h¸I¶¹®  ÐtÔ A u“Cc€Â¹ƒ®ÁQ}8¹ &ÿs?‰0Í¥3‚
s4È|çz=3ù3ó¾¬êëúú»=ÊÏ¯òÔîVÀþ¤
ñ©#©Ã~ÇýUz@†
Ü’Û?†G
«?¨r�/rº–Hè¯W�uÞ×ù»šê†¦P£Æ¬>h)Ua8j]U©s–¸�b"Ò�"KýÿM˜Õ66íi-5ô"KýÿM˜Õ66íi-5öKóþ^óöüÿ—¼ý+ÏÚï?Ø8yjªš˜Ñóî>á9¯E$„ê¨ù¨ÕS¿:&oj°nQl«6Q�£sB»ÃÎ€�æ°æuÑÖÃŽá�<ŠðâÓ4?
ê¢Ô|~/þ�Ó0æÈ8=Ç+èÍ8ŽÇ(·…0ƒŽhR<A»âÛ·èï�Romƒ†5Œ0ãg:ÃŸ�ÚÇßSÛ6ÄÄÜžÁ�O¢ÆÐa¸Q6ÐÌôñx¬ô;PÌØüpÄ²È`Æ>:<Y¶`óªàklxƒÃ€ˆ0|æÐvL çò£… Ã‡Î¾Èêp›<ïÆ³LÛ>¹³ë¡Ä®¸L¸±X|}LsõøöÜF¬kß<úæ
¬Ê¯ÏïLÀ¶8ºÏßõè©£N™`§VTÖM·V­Õ•6ª­ãk’ˆ*/W¶Ö&é¶.&|±û“‘:–Ø-“Š¾"¢›ÎÝkÝ×gÁM•"Tø>XHN^Ý¥£(ÎÞ?:½,~,�N6–Ã×3 D0¨É 3ºó¯³·‡³Œì,¬8Ã	´ííPÂYÂ›ÝÐ\ÐT]ˆMT=5PôÉU2Žª½�ï?þM·Ulsµ-ƒüteF}«ÿ�NþÈÎïŠñþ<éðgâóêïëLƒ:»díÎ$&í+�²³½Nˆ�Åÿò´ü|ã>›Â^lºõü%Ç¦Ïx4ÓÞ	tM˜�Â9Ñ+¡H ùyÃ�äQtš‚n3¸áOJ~°UD “ºÃWWß8$Âª¢£SMs4UTµo¨n«œáÎÒÅ^Ü¥ªamUë~Fú¥iU…*•µ¹Rfa%z‡J}c¥´„7æí1¸¹«+od£áïòÈ‘—œg§Ûƒý#�aŸ1ÜíðïF¦ÓY_OOÄÍ‚ø×©¼ûÛÒæ–Š<9RJÏJY*3\¢�hçCl‚-ÎË*	ƒ­­;Â¿\mC�†ÿØ¢5U0¤ï¬/¼l¢ÑIJ4Šmí‚˜4Þ6Zîž-�o%ê¸‡‚{_RÚáH˜ÃŽèü2ÖpgÌ÷dÑz•Hí¶�W+¬{µéóŠ½+—÷²7õáºÓn©êßL«€„Õ]V<ÕT”wb…žÙî¾ªÖi¡M©´
h»a0gf¡bjEVWV@+ÌÝ«üU#ß‡  ¬  	œ  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  G<?xml version="1.0"?><Features nextValidID="17"><Feature recentID="16" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6766" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5975-6766" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6767-6835" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="Neo" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3679-4473" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5219-5804" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="13" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2841-3269" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="12" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="235-614" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3283-3612" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2571-2795" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="615-818" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3463-3598" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4647-4768" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6836-6940" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="3xFLAG" directionality="1" translationMW="2730.72" type="CDS" allowSegmentOverlaps="0" cleavageArrows="2533" consecutiveTranslationNumbering="1" maxRunOn="2536" maxFusedRunOn="2536" detectionMode="exactProteinMatch"><Segment range="2468-2533" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;three tandem FLAGÂ® epitope tags, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment name="-10" range="4865-4871" color="#ffffff" type="standard"/><Segment range="4872-4889" color="#ffffff" type="standard"/><Segment name="-35" range="4890-4895" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4910-4931" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="863-881" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="1" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4841-4857" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="Cgas" directionality="1" translationMW="58195.28" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2536" maxFusedRunOn="2536"><Segment range="941-2461" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ?<Notes>
<UUID>a6e8cbc2-d930-4a6a-be3f-02fc170a5604</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.6.5</Created>
<LastModified UTC="12:6:8">2023.10.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  <StrandColors><TopStrand><ColorRange range="934..939" colors="green"/><ColorRange range="940..2460" colors="black"/></TopStrand><BottomStrand><ColorRange range="934..939" colors="green"/><ColorRange range="940..2460" colors="black"/></BottomStrand></StrandColors>  (     ˆ  ¸        ¸¿þ`�îÒu¸îód¥=&ã¿ŽˆŠ,á$ŒÄÕjS�uC¡ñÖ�…ã¤
C*Œã¬Æ'Ñ™Ì¡Úhy)¥’[Zpi+dý™�+Ì§eéÂVÏƒ‡Ï×üý¡ËYaÉŠý’\õ�¯hrÖšœ%xýè4�µ"m˜õ'?ù¡ËXp•ƒóàÚcô�‡é€z¾z'nri-³š}‚Â¨4�µ€È0¨{p¯RïÚµ€JŠ ßUµÀz½V,—^óý¡ÍX$2'.fSDîàåäEýã§ƒ¦ÅigÞdSƒ“*W¥:	ñ;óßcÙï_�½=¿pý·Îõf$þÛ«‚O†êPo}[w¸R~Më~O³qzž(U~ã‹ƒ…ˆö/KÐŸ@Óž#3ãàÿox˜9†¹æË¦®jûÕwŽ™ûJ*®îe/å6;à>´N‡½0Rî4¸…E)X£÷ƒà¶yÚz0¡º}½Á¼X­m9ÙuØí �<Qâvû«Úˆ>~$¡ÿ]¬kÄf8ÿMWl|ŽµÀ©¯]2ç®\=42âo�'=ÿèÑþ*=‹tõN´°l:C•î¸ðw¡o�F/ñ.ÅeãÏ¶‹ŸQtpWJ»x»AïÚËAEPA
}…�ý‡í ß¾þòx¿Q=G¾ÛH&Óîýï7‡í¾ÄuGØp†™Q¶±çPaÆI!ò¥8m>Çˆúä£ÓÛŠ*?ïü�¯¡þ÷¿x¾¯–U„£lÚ.B-®k€ €45 hdÐØà0€î`ë„0T_N î@	¿ÜÏâL3iLÃ ‚œÍ29Þ�LþLüï«:ºþ¾®Ïr³Ã«üµ;•°¿©¼jHêpß±ÿU^†�!‚·$€öÏá‘ÂªÏê KÜ®¥’º+Õà]wµþnÂæº¡©”¨ñ«š
UXN—Uj\Å¥® Xˆ´¤�RÿC¿ÓCE&5M�»ZKM}�RÿC¿ÓCE&5M�»ZKM}’üÿ—¼ý¿?åï?Jóö»ÏöZª¦¦4CÄ<û�¸NkÑI!:ª>Cª5TïÀN‰›Ú¬†ÔGA„*Í†…Ô`hÜÐ®ðó  D9¬9‡€Ýtu°ã¸cÏ"¼8´Í ÏÂº¨µ‹Â?……ãAÄ4Ì9²qÀÊú3N#±Ê-áLàãš� Ðnø¶íú;ãÄT›Û`á�c8ÙÎ†ðçãö±÷ÔÅvÍ±17'°`SÂè±´nM´³=<^+=Ô36?1ì²Ø1�Ž�m‚X<ê¸Û ðà"9´“(9ü†háDè0Å€áó¯²:œ&Ï;ñ¬Ó6Ï®lúèq+®î ¬VSý~=·«÷Ï>¹…B€ë²«ÀsÃ�†ûÓ0íŽ®³÷ýz*hÓ¦X)Õ•5“mÕ«ueMª«xÚä¢
‹Õíµ‰ºm‹‰Ÿ,~ääNƒ%¶dâ¯ˆ…h¦ó·Z÷uÙÄðSeH•> �–—·ihÊ3·�Î¯K_‹#Ó�¥…°õÌÀ*2hî¼ëìíáìã;+0ÄÂm;{T0–p¦÷E´—4bUMT=òUCÌ£ƒ*¯d;Ïÿ‡mÕ[íKD Å™QŸC*ÿàS¿²3»â¼�:|ø¼ú»úÀS Î®Ù;s‰	»JÂ$,¬ïS€â#qü­?8Ï¦ð—›.„½	Gqé³Þ4÷‚]“DÄ&$0ŽtJèR>^pçy]& ›Œî8SÒŸ¬Q($î†ðÕÕ÷Î	0ª¨¨ÔÓFœÍUíEêªç ¸s´�qW·)@j‚ØF›UAºß‘¾©ZUaJ¥mnT™˜I^‚áÒŸXém!ù»Ln.jÊÛÙ(ø{ü²$eçÀ@éöàÿHãXgÌG÷;|;Ñ©´ÖWÓÓÁ@ñ3E ¾5êo>öô¹¥¢�ND”’³Ò–JŒ×(£Ú9ÐÛ ‹s²Ê‚`ëkNð¯×PÄ§a¿ö(�UL);ëï(´RRƒÍ"›{`¦7�–»§‹cÛÉF:®!à„ž×Ô¶¸E’&0ÇÄ£º?µœYó=Ù4^¥R;m UÊë Å^íAº|â„oJÆ%ýì�ýxn´Ûªz·Ó*à!5WACU�5UeÁX¡g¶{¯ªµšhSjmš.ØLÙ¨Xš‘U•Õ�
ó7jÿH÷áË  ¬    !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  2<?xml version="1.0"?><Features nextValidID="17"><Feature recentID="16" name="Sting1-m" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2149" maxFusedRunOn="2149"><Segment range="941-2074" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="15" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4454-4470" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="863-881" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4523-4544" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment name="-10" range="4478-4484" color="#ffffff" type="standard"/><Segment range="4485-4502" color="#ffffff" type="standard"/><Segment name="-35" range="4503-4508" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="3xFLAG" directionality="1" translationMW="2730.72" type="CDS" allowSegmentOverlaps="0" cleavageArrows="2146" consecutiveTranslationNumbering="1" maxRunOn="2149" maxFusedRunOn="2149" detectionMode="exactProteinMatch"><Segment range="2081-2146" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;three tandem FLAGÂ® epitope tags, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6449-6553" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4260-4381" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3076-3211" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="615-818" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2184-2408" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2896-3225" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="235-614" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2454-2882" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="2" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4832-5417" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="1" name="Neo" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3292-4086" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6379" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5588-6379" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6380-6448" color="#ccffcc" type="standard" translated="1"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  @<Notes>
<UUID>9ed6fcca-e570-458d-9323-0dc9ff6f2e33</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.6.5</Created>
<LastModified UTC="12:1:16">2023.10.19</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
   «<StrandColors><TopStrand><ColorRange range="934..939" colors="green"/></TopStrand><BottomStrand><ColorRange range="934..939" colors="green"/></BottomStrand></StrandColors>  %d      p  v         v¿þ`�îÒu¸îód¥=&ã¿ŽˆŠ,á$ŒÄÕjS�uC¡ñÖ�…ã¤
C*Œã¬Æ'Ñ™Ì¡Úhy)¥’[Zpi+dý™�+Ì§eéÂVÏƒ‡Ï×üý¡ËYaÉŠý’\õ�¯hrÖšœ%xýè4�µ"m˜õ'?ù¡ËXp•ƒóàÚcô�‡é€z¾z'nri-³š}‚Â¨4�µ€È0¨{p¯RïÚµ€JŠ ßUµÀz½V,—^óý¡ÍX$2'.fSDîàåäEýã§ƒ¦ÅigÞdSƒ“*W¥:	ñ;÷’ôˆ ZãFoMÒ<ìAçT}‡i•aÛuaÔ’?*Wó†Óìx�®J==¸¢£þÿÈúúàP�{÷‹êùeXAZ6Í¢á$"Úæ¸  AÓP€QÖMŽ cæ¸CEôàä ›ýÌþ$Ã6”Ì0")ÌÓ!ó�èôÏäÏÎú³«¯ëêì÷+<:¿ËS¹[ ;ú�+Æ¤Ž§ûõUèi+rHlþ*¬þ¡Ê½ÊêY {¢½^×{_æì.kª™@j�°ù ¥U„á©uV¥ÌZZâˆ‹J@ˆ/ô;ý44RcTØÛµ¤´×Ðˆ/ô;ý44RcTØÛµ¤´×Ù/Ïù{ÏÛóþ^óô¯?k¼ÿ`áåªªjcD<CÏ¸û„æ½’Cª Cä:£UNüè™½ªÁ¸mDtA²¬Øh]F�Í
ï:CšÃ˜x×G[;†<ò+Ã‹LÐü+ª‹Qñø¼#øX^4CLÃ› à÷¯£4â;¢ÞÀP9 1Hðï‹nß£¾<EI½¶Ö0Ã�œèo~?k}LWlÛr{<.‹A†àqDÛ@;3ÓÅâ³ÐíC3cñÃnË!ýƒøèñfØ UƒÎ«�­±â Áó›AÙ2ƒŸÈfŽNƒX:û#©Âló¿Í3lúæÏ®‡ºá0à
Åañõ1Ï×ãÛq±¯|óë˜T(°[*¼<8o½3 nØàJë?×¢¦�:e‚�YSY6ÝZ·VTÚª·�®J ¨½^ÛX›¦Ø¸™òÇîNDè2[`¶N*øˆVŠo;u¯w]œO6T‰Sàùa!9{v–Œ£;xüêô°ø²=8ÚX[\ÌÂ£&€ÎëÎ¾ÎÞÎ3°²°ãL&Ó·µC	g
ot[@sAQv!5PôÕCÐ_%T<Ê82ªöC¼ÿøq6ÝU±ÎÔ´BWñÐy•ô2¯þ;û#;¾+Çøó§ÁŸ‹Ï«¿¬2êí“·8�›´¬"BÊÎõ8"7ÿÊÓñóŒúo	y²èK×ð”w›=àÓOx%Ði4LBbCçD®… ƒåçw‘EÒj	¸Îã…=)úÁU‚Nèo]_|à“
ªŠ�M4iÌÑUPnÔQ¾¡º®p‡;H{r”¨-„iµT­ùê•¥VªVÖåI™„•è.)õŽ–Òß›´Æâæ¬­½’�‡¿Ë"F^qœŸnôŽ5†|Äs·Ã½›Me}=<4Rã^¦óïoKšZ(ðäII+=)d¨ÍrŠ=£�²·;,¨&¶´ï
ýqµJvÿbˆÕTÂ“¾°¾ñ²‹E%(<Ò)·¶
`ÓxÙkºx¶=¼”cªâIí}Kk„Y"c|J;£ðËYÀeŸ3Ý“EêU#¶Ú\®°UîÔ§Î(Fô¬b_ÞÈß×†ëMº§«}2®Ut5XóUPP1ÜŠ{gºú«Y¦…6¦Ð)¢í„Á�š…‰©Y]Y ¯3v¯ñT�~  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  á<?xml version="1.0"?><Features recycledIDs="16" nextValidID="17"><Feature recentID="15" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="3364-3380" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="863-881" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="3433-3454" color="#31849b" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment name="-10" range="3388-3394" color="#ffffff" type="standard"/><Segment range="3395-3412" color="#ffffff" type="standard"/><Segment name="-35" range="3413-3418" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="3xFLAG" directionality="1" translationMW="2730.72" type="CDS" allowSegmentOverlaps="0" cleavageArrows="1056" consecutiveTranslationNumbering="1" maxRunOn="1059" maxFusedRunOn="1059" detectionMode="exactProteinMatch"><Segment range="991-1056" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;three tandem FLAGÂ® epitope tags, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="10" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5359-5463" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3170-3291" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1986-2121" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="615-818" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="6" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1094-1318" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1806-2135" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="235-614" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="1364-1792" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="RIGHT"/></Q></Feature><Feature recentID="2" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3742-4327" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="direction"><V text="LEFT"/></Q></Feature><Feature recentID="1" name="Neo" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="2202-2996" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature><Feature recentID="0" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="5289" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="4498-5289" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="5290-5358" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ><Notes>
<UUID>4ff05e09-b66c-469c-8065-75f8cff75ce8</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="16:0:0">2017.6.5</Created>
<LastModified UTC="9:9:55">2022.7.15</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Pý7zXZ  æÖ´F !   t/å£à€¹] Ë‡ØÎf‘ƒÊý{3Ôé·Ú(1v%f M*	mj÷)p80çÌ*iÚ	´NY]Qó“�úF7î&ƒÕ!Ò÷¿Û{ùï/U´
[¦0é«‰8`�©V‡ˆìFØ¼Òwk£»è(Ñ[	Î0­"{}†-Dr¤Bcé¶~‚"Ñ=ûFßl»#ßòsKÇ€~ va€À•q´­Õn²¹CGŒiž’¿²†Íy.ã0"’Í#W”ÊÈäC²Ve;Œs³¨O†¤j‡þvs±ã¦»Öš˜ïD2s{ ñ¾ñd!ðÜ[µâ>}òŸÃ9oÖÅûs°$ƒ@±Uv÷§°ÌfùÞ~Üð¦ÐšÇVhºì²¯ùâè�PïX«6HLŸy§Áw×ŠãùnÂ¤7ÙÖÐˆ;«jæè¤„:O£/Œ¸î7—�È–¸·<z‰Ù·sÌM3‹ÖÅ�DVÈ¡�8@Ï±C³=Êï`}ÖÛ-Ë´�G1mÊ5®±÷1Fu û¢¶IL“»Lo“à}±u¥©S9¸”Œæ‚Èº„÷ÞÌ?ô—G‡msuš+‡7”Qî.tLgWÆÔÜ d õ·W[ƒ‡ßd@–:Q¦˜@">¡~át‚�R'å7mŸ ù¢B–»ËÒÔ�a:“î M¼�ëÆx Â„WéjùëmG†TÄZ…‰*{Ç;ƒ�“º‘ú·kO¤‡ØÄ-ô¾ewbÑ8éš}tK•>œ4ù'HºòH3É³ŽA3ƒ‰>rYŸˆsœ8…PDi«ïÞýØŽ/ÔG‹Ls	Ê ó§"ý`Ÿsš¥ã×´ž"aÔqH[«ã™Ë�Â=åAæ–Ú/‚¼:ø¢LM±çZó//øcðœÆþ@™½ä¨xß?|á/Ÿ—r’ó4�Ó`'µ*ÞÎ\ñÒS€„2B ’;$²…'¦âm‡zvféê¨J¹;Vå²ˆÑ¹n@íÈgú�÷ÉÃ�9Ç­Úi v®÷vì9»\Îx’C‡.¼mƒmƒ¼c®ËÙï*”pý¶�>÷˜¼p<3SjÍÕJ4COvS»â¬(¸àh×Ù�³Që$8‚"f%ÄkÿHKãB3«Ð»óÅt :yu16j×m) S¨m‘�œ¸…ÎêHóžçê ÿéSÊøÁÊ‹/û((à«.Ÿâ¥â¬¬&dzS yÿ
¢yŠÄïãÏ‡Öõ´¹#3Ù)pd¤@Þ³{�úÆ5ún´	ÏÑ«üÀ›ü§ÛPMÑVÚ,^¥‹¤ô 9ëðœ#â�ä÷n~ÈtOµ°nŽ£ÍrtïWJ³ÀŽ6ç‘¢¯\‘ôOswNÓóÁ¦ü»&ù�k®È¨#×Èhê&Ôˆ1wmäN“¢cêÌ3š‡kW‘6Ü¡Ö—<ÿLö¹ßÄÏ[3ª
BÑw·>ÈxŸ;ŒÚ¶¬ÛM¥òFjPÕt„>à4ç‘ÙÜ…D¥¤Ñé[áMÂê$í`­€Bf¸â§‰o(-ã:…Ô�ÊL*0`¿,s,\†ìPø¤ú¬Ç}Oòl	]0Ç…ŒoÊ,>c�Ü­ä¿4þâÁáaþ�©ž$FTG†L2©>Ïƒ‡ò½fr 5ìa™ux€ÛÒ‚7ÛPðŸvGû¡ˆèü®Æhþzë+9¾SÅ¤ =«ñjìft,]$ÚñÃ�­ZF²O”~ïÕÐe›N¯×ÒaÖéDš™üPà*†v÷Ùf’6¾â±#�È H³µ©ñ/FÎŠ¦¹:3ÀëÿkÀšñüÐm‰†Ñ´Õ(&Ï6Eß¼Ø=öc@ô‡‰Œ	Ð‘ïó&Fˆbå€ !,S™G”ÖfQ
_C¬¦`]ÔM…¥§r�M
'
@}ÍÐ
×F7äQfÔ‡Gþì�…‘×ÉëÙ•zyQª³©@²½†@ã@ÔBžçãÝBF|³–¹å£³W;®ËÐv
pXx•àÄInIzèã®NãG8”`2ÁÇŸµe±ëó‹Á?Ïà–Ýã¬Gàv©=@Àï?ÄÀ+óójô]ß¯µ"¼I´~­›‰Ë\L"GéÜs=ùPç:ù—ü_l×Ö±gêêmÏ
w Áøi™JÝÉ�±÷ÞÕnêáÝ‰ÛxUX®L· _n!wÚDüÒÚìW0ñ Kdê2ÔØíjsƒÊÛ_VyË–Œd d"÷Q�AtbÖ”w'B”)¾u¦ 3Ëø�¾éDªÌ*½.ATÛ3™Q$ÌÎé·ýÚ"‰›Ò;ˆž:Ä%ù¸T@^ by‹ G6f¿•pjY·*›9ÑFBCJWÛ¾bóP·CÕîÖkƒ«€O‹àCÒük€±g¦¿Âj…º^ úpoå¤HNF“vì~¼9�á+E¦ÊE 8ÁÎš©“	Xá{b()„eÔ¡¡�±ÖÓâFÙ	ÒÇK&°ÉaßäH+óóÒhèm~!:XúRR�Y´4q3$Ñþ:ü<hgP«™s„¢r<f¼>$Û~ýd¥8WP;À6óc~5ãðÔ–ävšÀÝ(¤=èóHXæã�,;¦¼DÍàª»ïÐ¡o½Óÿ.ÍŒ�Ë¥[¯DþÛ‘nÕV~j´‚…*ÍÛ³¦æ]¸U7Ñ§}ÓOaö–¦ý�/¯Ðë¢X¦sÏÏ?]“2L�íÓ8­²L½{j^-eEj¸ö
$ÝwZ­z"`¥$`8X„YlY}„`ÒTÁ� R0ËO‚¢–Î-âeÝÔúüüé€ªe[½àº.Æ$‡¸à|¾3fõ%¬í,2¤jqIcF%°ƒ:–?K9ß?ØýI ó6sE�f ÿ>>#z4ÐF Û"Ëˆuœ:WGÀX…¸˜ºÂ|{9.*��ÚT=#ÇÎ§ˆçNšÕ)æRŽ(åQRã`#»õt/ÇFÏÝy´¾‘ä?­³!¸M”9€M1^õúUÓO7’·;wR7Ôe`ósŠpÍ›ÏlKC�Ë‘§EóŽškÉžpÕYßùdÌwœ�&Š­íÀß¶¨ÈÜNo}ºè]E™).ÕÔ];¿Mrµó³GBªƒo‹pÕZË€èt;ª¹ 	1H&Ø9EÚ'ö›Zi@"ü¾z\D59‚¥~þØ.ñ3gL¿)v�ŸÌÉñ…Ë°)2vŒºH{¦.00­K)ÔãÎ½�â÷VÇh§àü²pD¶ÛLÌïª¢³¬ÊHPüÄ®÷¿NT^‰Ža…TmœÛ ¯Ú¿Æ4ŽâR“C~†€¯ÝFå“’z…ªR#Ew† DQ»~Ó×º�…õ1i+!ƒbØÁ&vØÐRËv¯]SìÏ $!ÁUaqÓð½’»Sg²ùÂh�¦—xUT•x¹�,«ÕJÓv…<KGP¯.Yµ;§�Ü«í,å(«ª˜Š”ƒo¤úK¨“	²Êàkÿ1ü4ý8k’¹—©	…°fy‘¥u‘+ýTBžÁ—‰ÇÅC¨E�å&Ön¹hey…V>–a+Ñ|×"F€	Ž›¼ý%)rhý t¶OØd¾oô«t³MåŸ=îA€œÄZÉ£úŠé�
 F˜ÖSÖ1Š•(j7ö}à·4éìIDù‹lçÞ˜´8ÄfsyD`°G„¬yê„“/aœªaP�¨N¿$G€u±lL%=¢F<‚˜j]ÑUžô¦@òvíRêe|ðY*5W
þ.n5åeeô\HRv1FQ¯tCA•Ì t^ÞãœâÐx3æ°2»„õžžYØóÊàî‡?BmùW×œIeÓâô�·;AFŠÕàZC—­Å³7I�ÅT+®'ð°ê¾�™
’¾ž‹:d¹dyú3 Nø$PôvU<c:°“åA<µÝO%‰²�—G��e²œÈ·Y�§·é84u_pÎ‹áðV5OÂUÁÈÊ•Ì^1XMjÔÍóÑ¢Øðw÷v=}U(Ê“-ý‡”#m Û¢È%#÷nµyfW·I-gÑZAA¡-ËO»_ÐÆ]¸âò¸¼¸]k1´—ÙQÿÉ`( ®gÃ¥D£c%ùŠÖôÆzDÌ‹?æÍ©Ÿ2O´ìT C¦ä«ð‚²c }Ž'™§YÙi»@Bnl-$Íì£!"‡wåì�ðfŽõ.»–Ëøiõ!ÍqøÜi|¨áõb?ïüh'µÚáa�´P&ÐbQæ{måT×e`ÞOíÈx—²Àç¸iÈê9L�\Ç»Žm29ryq/± æ‚í+DÛ}K¾©ÝŸ ØØçVõHŠ$S3±/(ÂF³¦\«QÉ¡…­X!€3›Ð„`Ú¨=LÑYæÕ¨6\«:ÔTâw—:Îã‹«›ØuïÈ*Ö„èÙHI©5õE²�±Ó™‰xP„4ð
Ôº¤LÅØM°<íË¤HÍ€Àd¾Š¤�}î0  ¼bír+d ¬º�  7Õ'±Ägû    YZ   -<AlignableSequences trimStringency="Medium"/>  !<AdditionalSequenceProperties><UpstreamStickiness>0</UpstreamStickiness><DownstreamStickiness>0</DownstreamStickiness><UpstreamModification>FivePrimePhosphorylated</UpstreamModification><DownstreamModification>FivePrimePhosphorylated</DownstreamModification></AdditionalSequenceProperties>
  #<?xml version="1.0"?><Features nextValidID="17"><Feature recentID="0" name="IRF7" directionality="1" translationMW="54279.32" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="2524" maxFusedRunOn="2524"><Segment range="941-2449" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MALAPERAAPRVLFGEWLLGEISSGCYEGLQWLDEARTCFRVPWKHFARKDLSEADARIFKAWAVARGRWPPSSRGGGPPPEAETAERAGWKTNFRCALRSTRRFVMLRDNSGDPADPHKVYALSRELCWREGPGTDQTEAEAPAAVPPPQGGPPGPFLAHTHAGLQAPGPLPAPAGDKGDLLLQAVQQSCLADHLLTASWGADPVPTKAPGEGQEGLPLTGACAGGPGLPAGELYGWAVETTPSPGPQPAALTTGEAAAPESPHQAEPYLSPSPSACTAVQEPSPGALDVTIMYKGRTVLQKVVGHPSCTFLYGPPDPAVRATDPQQVAFPSPAELPDQKQLRYTEELLRHVAPGLHLELRGPQLWARRMGKCKVYWEVGGPPGSASPSTPACLLPRNCDTPIFDFRVFFQELVEFRARQRRGSPRYTIYLGFGQDLSAGRPKEKSLVLVKLEPWLCRVHLEGTQREGVSSLDSSSLSLCLSSANSLYDDIECFLMELEQPA"/></Q></Feature><Feature recentID="1" name="lac operator" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4829-4845" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;lac&lt;/i&gt; repressor encoded by &lt;i&gt;lacI&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;The &lt;i&gt;lac&lt;/i&gt; repressor binds to the &lt;i&gt;lac&lt;/i&gt; operator to inhibit transcription in &lt;i&gt;E. coli&lt;/i&gt;. This inhibition can be relieved by adding lactose or isopropyl-Î²-D-thiogalactopyranoside (IPTG).&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="2" name="T7 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="863-881" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for bacteriophage T7 RNA polymerase&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="3" name="CAP binding site" type="protein_bind" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment range="4898-4919" color="#31849b" type="standard"/><Q name="bound_moiety"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;E. coli&lt;/i&gt; catabolite activator protein&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;CAP binding activates transcription in the presence of cAMP.&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="4" name="lac promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" visible="0"><Segment name="-10" range="4853-4859" color="#ffffff" type="standard"/><Segment range="4860-4877" color="#ffffff" type="standard"/><Segment name="-35" range="4878-4883" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;promoter for the &lt;i&gt;E. coli lac&lt;/i&gt; operon&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="5" name="3xFLAG" directionality="1" translationMW="2730.72" type="CDS" allowSegmentOverlaps="0" cleavageArrows="2521" consecutiveTranslationNumbering="1" maxRunOn="2524" maxFusedRunOn="2524" detectionMode="exactProteinMatch"><Segment range="2456-2521" color="#cc99b2" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;three tandem FLAGÂ® epitope tags, followed by an enterokinase cleavage site&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="DYKDHDGDYKDHDIDYKDDDDK"/></Q></Feature><Feature recentID="6" name="AmpR promoter" directionality="2" type="promoter" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="6824-6928" color="#ffffff" type="standard"/><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="7" name="SV40 poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4635-4756" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="8" name="SV40 ori" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3451-3586" color="#ffff00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="9" name="CMV promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="615-818" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus (CMV) immediate early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="10" name="bGH poly(A) signal" type="polyA_signal" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2559-2783" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;bovine growth hormone polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="11" name="SV40 promoter" directionality="1" type="promoter" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="3271-3600" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 enhancer and early promoter&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="12" name="CMV enhancer" type="enhancer" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="235-614" color="#ffffff" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;human cytomegalovirus immediate early enhancer&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="13" name="f1 ori" directionality="1" type="rep_origin" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="2829-3257" color="#ffff00" type="standard"/><Q name="direction"><V text="RIGHT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="14" name="ori" directionality="2" type="rep_origin" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="5207-5792" color="#ffff00" type="standard"/><Q name="direction"><V text="LEFT"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;high-copy-number ColE1/pMB1/pBR322/pUC origin of replication&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="15" name="Neo" directionality="1" translationMW="29048.78" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" hitsStopCodon="1"><Segment range="3667-4461" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;aph(3')-II&lt;/i&gt; (or &lt;i&gt;nptII&lt;/i&gt;)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to neomycin, kanamycin, and G418 (GeneticinÂ®)&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;aminoglycoside phosphotransferase from Tn5&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF*"/></Q></Feature><Feature recentID="16" name="AmpR" directionality="2" translationMW="31558.16" type="CDS" swappedSegmentNumbering="1" allowSegmentOverlaps="0" cleavageArrows="6754" readingFrame="-1" consecutiveTranslationNumbering="1" hitsStopCodon="1" detectionMode="exactProteinMatch"><Segment range="5963-6754" color="#ccffcc" type="standard" translated="1"/><Segment name="signal sequence" range="6755-6823" color="#ccffcc" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="gene"><V text="&lt;html&gt;&lt;body&gt;&lt;i&gt;bla&lt;/i&gt;&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="note"><V text="&lt;html&gt;&lt;body&gt;confers resistance to ampicillin, carbenicillin, and related antibiotics&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="product"><V text="&lt;html&gt;&lt;body&gt;Î²-lactamase&lt;/body&gt;&lt;/html&gt;"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MSIQHFRVALIPFFAAFCLPVFA,HPETLVKVKDAEDQLGARVGYIELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLIKHW*"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ¦<Notes>
<UUID>fed03416-4d48-4981-8870-5810d3e78c93</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pCMV-IRF7(human)-3Ã—FLAG-Neo</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="16:0:0">2017.6.5</Created>
<LastModified UTC="11:46:23">2025.7.9</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         
?????                                                                                                       ÿ d      DO  d               E              ÿÿÿÿYô? P                                                                                                                                                             3<StrandColors><TopStrand><ColorRange range="916..933" colors="black"/><ColorRange range="934..939" colors="light blue"/><ColorRange range="953..960" colors="magenta"/><ColorRange range="961..970" colors="light blue"/><ColorRange range="1266..1266" colors="magenta"/></TopStrand><BottomStrand><ColorRange range="916..933" colors="black"/><ColorRange range="934..939" colors="light blue"/><ColorRange range="953..960" colors="magenta"/><ColorRange range="961..970" colors="light blue"/><ColorRange range="1266..1266" colors="magenta"/></BottomStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
